Conjugation

Part:BBa_K2759003:Design

Designed by: Mehmet Ali Hoşkan   Group: iGEM18_Bilkent-UNAMBG   (2018-10-09)


Bacterioferritin (BFR) with sfGFP conjugation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 106
    Illegal SapI.rc site found at 499


Design Notes

Conjugation of BFR with sfGFP provided us a visualization method for the cells that ar expressing BFR so that the visualization of the bacteria would be so much easier. BFR stop codon is excluded from the coding region since our goal is to obtain conjugate protein.

Source

The source of BFR is iGEM14_Berlin Team, from iGEM distribution kit 2018 and the source of sfGFP is the Synthetic Biosystems Lab that we are working in UNAM.

References

Yang, Xiaoke, et al. "The iron oxidation and hydrolysis chemistry of Escherichia coli bacterioferritin." Biochemistry 39.16 (2000): 4915-4923.

Baaghil, Suzanne, et al. "Core formation in Escherichia coli bacterioferritin requires a functional ferroxidase center." Biochemistry 42.47 (2003): 14047-14056.